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阿拉丁核酸合成试剂

2021/5/31 15:59:15  作者:阿拉丁试剂


 

阿拉丁核酸合成试剂
 

      寡核苷酸是短的、单链或双链的DNA或RNA分子。其合成方法发展历程:从Michelson和Todd早期的H-磷酸盐和磷酸三酯法(H-Phosphonate & Phosphotriester);到1950年代Khorana的磷酸二酯法(Phosphodiester);以及1960-1970年代的磷酸三酯法(Phosphotriester)和亚磷酸三酯法(Phosphite triester)的发展。而直至1980年代,Marvin Caruthers的亚磷酰胺法(Phosphoramidite),加上自动化固相技术,成为了目前主流的寡核苷酸合成技术方法。在聚合酶链反应(PCR)、核酸测序、基因检测、核酸药物研发等领域有广泛应用。

      亚磷酰胺寡聚体的合成在3'-5'方向进行(与生物合成中DNA复制的5'-3'方向相反)。每个合成周期添加一个核苷酸。亚磷酰胺DNA合成循环如图所示。

 

亚磷酰胺DNA合成循环

保护核苷

产品号 中文名称 英文名称 级别 Cas 规格
I119475 N2-异丁酰基-2′-脱氧鸟苷 N2-Isobutyryl-2′-deoxyguanosine 98% 68892-42-2  100mg,250mg,1g,5g
I119519 5'-O-(4,4'-二甲氧基三苯基)-N2-异丁酰基-2'-脱氧鸟苷 N2-Isobutyryl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosine 99.00% 68892-41-1 1g,5g,10g,25g,100g
N159691 N6-二苯甲酰基腺苷-2',3'-二苯甲酸酯 N6-Dibenzoyladenosine 2',3'-Dibenzoate >98.0%(HPLC)(T) 58463-04-0 100mg,1g
B119471 N4-苯甲酰基-5′-O-(4,4′-二甲氧基三苯基)-2′-脱氧胞苷 N4-Benzoyl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxycytidine 99% 67219-55-0 1g,5g
B103100 N4-苯甲酰胞苷 N4-Benzoylcytidine 98% 13089-48-0 1g,5g,25g
B119532 N4-苯甲酰基-2'-脱氧胞苷 N4-Benzoyl-2′-deoxycytidine 98.00% 4836-13-9 250mg,1g,5g
B122954 N6-苯甲酰基-2'-脱氧腺苷水合物 N6-Benzoyl-2′-deoxyadenosine hydrate 98% 206752-42-3,4546-72-9 250mg,1g,5g
B119470 N6-苯甲酰基-5′-O-(4,4′-二甲氧基三苯基)-2′-脱氧腺苷 N6-Benzoyl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyadenosine 99% 64325-78-6 100mg,1g,5g
O117684 5′-O-(4,4′-二甲氧基三苯甲基)胸苷 5′-O-(4,4′-Dimethoxytrityl)thymidine 98.00% 40615-39-2 1g,5g,25g
B119477 N6-苯甲酰基腺苷 N-Benzoyladenosine 97% 4546-55-8 1g,5g,25g

核糖,2'-脱氧核糖

产品号 中文名称 英文名称 级别 Cas 规格
R105972 L-核糖 L-(+)-Ribose 98% 24259-59-4 1g,5g,10g,25g,100g
T107915 四乙酰核糖 1,2,3,5-Tetra-O-acetyl-β-D-ribofuranose 98% 13035-61-5 5g,25g,100g,500g
R104821 D-(-)-核糖 D-(-)-Ribose ≥99%(HPLC) 50-69-1 5g,25g,100g,250g,500g
R104820  D-(-)-核糖 D-(-)-Ribose 98% 50-69-1 100g,500g,2.5Kg
R103230  1-乙酰基-2,3,5-三苯甲酰氧基-1-beta-D-呋喃核糖 β-D-Ribofuranose 1-acetate 2,3,5-tribenzoate 98% 6974-32-9 1g,5g,25g,100g
M158368 甲基-β-D-呋喃核糖苷 Methyl β-D-Ribofuranoside >97.0%(HPLC) 7473-45-2 1g,5g,25g,100g
D121077 3,5-二-O-(对甲苯酰)-2-脱氧-Α-D-呋喃核糖氯 3,5-Di-O-(p-toluyl)-2-deoxy-D-ribofuranosyl chloride 90% 4330-21-6 250mg
D104784  2-脱氧-D-核糖 2-Deoxy-D-Ribose 98% 533-67-5 1g,5g,25g,100g
D123989 2-脱氧-2,2-二氟-D-赤-戊糖酸γ-内酯3,5-二苯甲酸酯 2-Deoxy-2,2-difluoro-D-erythro-pentonic Acid γ-Lactone 3,5-Dibenzoate 98% 122111-01-7 1g,5g,25g,100g
T139496 2-脱氧-2-氟-1,3,5-三-O-苯甲酰基-α-D-阿拉伯呋喃糖 2-Deoxy-2-fluoro-1,3,5-tri-O-benzoyl-α-D- arabinofuranose - 97614-43-2 1g,5g,25g

硫转移试剂

产品号 中文名称 英文名称 级别 Cas 规格
X162986 氢化黄原素 Xanthane Hydride >95.0%(HPLC) 6846-35-1 5g,25g,100g,500g
H157193  3H-1,2-苯并二硫醇-3-酮1,1-二氧化物 3H-1,2-Benzodithiol-3-one 1,1-Dioxide >98.0%(HPLC) 66304-01-6 250mg,1g,5g
B152485 双(苯乙酰)二硫化物 Bis(phenylacetyl) Disulfide >98.0%(HPLC) 15088-78-5 5g,25g

磷酸化试剂,磷硫化试剂

产品号 中文名称 英文名称 级别 Cas 规格
M157887 二氯磷酸甲酯 Methyl Phosphorodichloridate >95.0%(T) 677-24-7 5g,25g,100g
C119480 2-氰乙基N,N,N′,N′-四异丙基亚磷酰二胺 2-Cyanoethyl N,N,N′,N′-tetraisopropylphosphordiamidite 97% 102691-36-1 1g,5g,25g,100g
T162687 焦磷酸四苄酯 Tetrabenzyl Pyrophosphate >98.0%(HPLC) 990-91-0 250mg,1g,5g,25g
P104277 二氯化磷酸苯酯 Phenyl dichlorophosphate 98% 770-12-7 25g,100g,500g
B152354 二(四丁铵)焦磷酸二氢盐 Bis(tetrabutylammonium) Dihydrogen Pyrophosphate >96.0%(T) 857447-79-1 50mg,200mg
H157193  3H-1,2-苯并二硫醇-3-酮1,1-二氧化物 3H-1,2-Benzodithiol-3-one 1,1-Dioxide >98.0%(HPLC) 66304-01-6 250mg,1g,5g
C135875 2-氯-1,3,2-二氧磷杂环戊烷 2-氧化物 2-Chloro-1,3,2-dioxaphospholane-2-oxide ≥95.0% 6609-64-9 5g,25g,100g
D156020 氯硫代磷酸二乙酯 Diethyl Chlorothiophosphate >97.0%(GC) 2524-04-1 5g,25g,100g
C154011 2-氯苯基二氯膦酸酯[磷酸化剂] 2-Chlorophenyl Phosphorodichloridate [Phosphorylating Agent] >97.0%(T) 15074-54-1 1g,5g,25g,100g
C154013 2-氯-4H-1,3,2-苯并二氧磷-4-酮 2-Chloro-4H-1,3,2-benzodioxaphosphorin-4-one >95.0% 5381-99-7 1g,5g,25g
D109475  氯代磷酸二苯酯 Diphenyl phosphoryl chloride 97% 2524-64-3 25g,100g,500g
M157888 二氯亚磷酸甲酯[亚磷酸化剂] Methyl Dichlorophosphite [Phosphitylating Agent] >95.0%(T) 3279-26-3 5g,10g,25g,100g
B152485 双(苯乙酰)二硫化物 Bis(phenylacetyl) Disulfide >98.0%(HPLC) 15088-78-5 5g,25g
C154012 4-氯苯基二氯磷酸酯 4-Chlorophenyl Phosphorodichloridate >97.0%(T) 772-79-2 5g,25g,100g
D110276 焦磷酰氯 Diphosphoryl chloride 97% 13498-14-1 5g,25g,100g,500g
C153709 5-氯水杨基-N,N-二异丙基亚磷酰胺 5-Chlorosaligenyl-N,N-diisopropylphosphoramidite >95.0%(HPLC) 1620086-77-2 200mg,1g
C154014 2-氯-1,3,2-二氧磷杂环戊烷 2-Chloro-1,3,2-dioxaphospholane >97.0%(GC) 822-39-9 5g,25g,100g
D138186 N,N-二异丙基亚磷酰胺二叔丁酯 Di-tert-butyl N,N-diisopropylphosphoramidite ≥95.0% 137348-86-8 1g,5g
D122720  二苄基 N,N-二异丙基亚磷酰胺 Dibenzyl N,N-Diisopropylphosphoramidite 98% 108549-23-1 1g,5g,25g,100g

缩合剂

产品号 中文名称 英文名称 级别 Cas 规格
N132858 3-硝基-1,2,4-三唑 3-Nitro-1,2,4-triazole ≥98.0%(HPLC) 24807-55-4 1g,5g,25g,100g
M121621 均三甲苯基磺酰氯 2-Mesitylenesulfonyl chloride 99% 773-64-8 5g,25g,100g,500g
C113337 N,N'-羰基二(1,2,4-三氮唑) 1,1'-Carbonyl-di-(1,2,4-triazole) 96% 41864-22-6 5g,25g,100g
M135672 2,4-二磺酰氯基均三甲苯 2,4-Mesitylenedisulfonyl Dichloride ≥98.0% 68985-08-0 5g,25g
T109597 特戊酰氯 Trimethylacetyl chloride 98% 3282-30-2 25ml,100ml,500ml,2.5L,10L
B115197 5-苄硫基四氮唑 5-(Benzylthio)-1H-tetrazole 98.50% 21871-47-6 5g,25g,100g
D106074 N,N'-二环己基碳二亚胺(DCC) N,N′-Dicyclohexylcarbodiimide 99.00% 538-75-0 25g,100g,500g,1kg,2.5kg,10kg
C153419 1-(氰甲基)哌啶四氟硼酸盐 1-(Cyanomethyl)piperidinium Tetrafluoroborate >98.0%(N) 434937-12-9 1g,5g
T113353 1-(2,4,6-三异丙基苯基磺酰)咪唑 1-(2,4,6-Triisopropylbenzenesulfonyl)imidazole 98% 50257-40-4 1g,5g,10g,25g
D109319 4,5-二氰基咪唑 4,5-Dicyanoimidazole 98% 1122-28-7 5g,25g,100g
D109320 4,5-二氰基咪唑 4,5-Dicyanoimidazole 99% 1122-28-7 5g,25g,100g
T161612 2,4,5,6-四甲基苯二磺酰二氯 2,4,5,6-Tetramethylbenzenedisulfonyl Dichloride >98.0%(T) 97997-76-7 1g,5g
T138965 1-(2,4,6-三异丙基苯磺酰)-1,2,4-三唑 1-(2,4,6-Triisopropylphenylsulfonyl)-1,2,4-triazole >98.0%(HPLC) 54230-60-3 1g,5g,25g
T106183 1-对甲基苯磺酰咪唑 1-(p-Toluenesulfonyl)imidazole 99% 2232-08-8 5g,25g,100g,500g
T109341 2,4,6-三异丙基苯磺酰氯 2,4,6-Triisopropylbenzenesulfonyl chloride 97% 6553-96-4 5g,25g,100g,500g
D105651 N,N'-二环己基-4-吗啉脒 N,N′-Dicyclohexyl-4-morpholinecarboxamidine 98% 4975-73-9 5g,25g,100g,500g
T100645 1,2,4-三氮唑 1,2,4-Triazole 99% 288-88-0 25g,100g,250g,500g
E111310 5-乙硫基四氮唑 5-(Ethylthio)-1H-tetrazole 98% 89797-68-2 1g,5g,25g,100g

羟基保护剂,氨基保护剂

产品号 中文名称 英文名称 级别 Cas 规格
I107549 异丁酰氯 Isobutyryl Chloride 98% 79-30-1 25ml,100ml,500ml,2.5L
H106017 六甲基二硅胺烷 Hexamethyl disilylamine AR,98% 999-97-3 100ml,500ml,2.5L,10L
D154192 二叔丁基二氯硅烷 Di-tert-butyldichlorosilane >95.0%(GC) 18395-90-9 1g,5g
N106603 2-硝基溴化苄 2-Nitrobenzyl bromide 97% 3958-60-9 5g,25g,100g
P160117 苯氧基乙酰氯 Phenoxyacetyl Chloride >98.0%(GC) 701-99-5 25g,100g,500g
B152896 1,3-苯并二硫代吡咯四氟化硼盐[羟基保护剂] 1,3-Benzodithiolylium Tetrafluoroborate [Hydroxyl-Protecting Agent] >96.0%(T) 57842-27-0 1g,5g
T109041 N,O-双(三甲基硅烷基)乙酰胺 N,O-Bis(trimethylsilyl)acetamide 95% 10416-59-8 25ml,100ml,500ml,5L
T131644  N,O-双(三甲基硅烷基)乙酰胺 TMS-BA 用于GC衍生化 10416-59-8 5ml,25ml
A108664 乙酰氯 Acetyl chloride 99%,无色 75-36-5 500ml,2.5L
A108662 乙酰氯 Acetyl chloride AR,98% 75-36-5 100ml,500ml,12*500ml,2.5L,10L
C134968 2-氯代三苯甲基氯 2-Chlorotrityl Chloride ≥97.0% 42074-68-0 25g,100g,500g,2.5kg
T109597 特戊酰氯 Trimethylacetyl chloride 98% 3282-30-2 25ml,100ml,500ml,2.5L,10L
D106289 N,N-二甲基甲酰胺二甲缩醛 DMF-DMA 98%,用于酯化 4637-24-5 25g,100g,500g
D106288 N,N-二甲基甲酰胺二甲缩醛 N,N-Dimethylformamide Dimethyl Acetal 97% 4637-24-5 25ml,100ml,500ml
T106184 三苯基氯甲烷 Trityl Chloride 97% 76-83-5 25g,100g,250g,500g,2.5kg
C153889  4-氯苯氧基乙酰氯 4-Chlorophenoxyacetyl Chloride >98.0%(T) 4122-68-3 5g,10g,25g,100g,500g
T109341 2,4,6-三异丙基苯磺酰氯 2,4,6-Triisopropylbenzenesulfonyl chloride 97% 6553-96-4 5g,25g,100g,500g
D109292 4,4'-二甲氧基三苯基氯甲烷 4,4′-Dimethoxytrityl chloride >97.0% 40615-36-9 5g,25g,100g,500g
D119481 二叔丁基硅基双(三氟甲烷磺酸) Di-tert-butylsilyl bis(trifluoromethanesulfonate) 97% 85272-31-7 1g,5g,25g
B111191 叔丁基二苯基氯硅烷 tert-Butyldiphenylchlorosilane 98% 58479-61-1 10g,25g,100g,250g
B103644  叔丁基二甲基氯硅烷 tert-Butyldimethylsilyl chloride 97% 18162-48-6 25g,100g,250g,500g
T162634 4,4',4''-三(苯酰氧基)三苯甲基溴[羟基保护剂] 4,4',4''-Tris(benzoyloxy)trityl Bromide [Hydroxyl Protecting Agent] >97.0%(T) 86610-66-4 1g,5g,25g
D113529 1,3-二氯四甲基二硅氧烷 1,3-Dichloro-1,1,3,3-tetramethyldisiloxane 95% 2401-73-2 5g,25g,100g
D139183 1,3二氯-1,1,3,3-四异丙基二硅氧烷 1,3-Dichloro-1,1,3,3-tetraisopropyldisiloxane ≥97% 69304-37-6 1g,5g,25g,100g
M100611 4-甲氧基苯甲酰氯 4-Methoxybenzoyl chloride 97% 100-07-2 25g,100g,500g
B104566 苯甲酰氯 Benzoyl chloride ACS, ≥99.0% (T) 98-88-4 500g
C104814  三甲基氯硅烷(TMCS) Chlorotrimethylsilane ≥99.0%(GC) 75-77-4 100ml,250ml,500ml,2.5L,10L
C104813  三甲基氯硅烷(TMCS) Chlorotrimethylsilane >98.0%(GC) 75-77-4 100ml,250ml,500ml,2.5L,10L
T106616 三氟甲磺酸三甲基硅酯 Trimethylsilyl trifluoromethanesulfonate 98.00% 27607-77-8 5g,10g,25g,50g,100g,250g,500g
M113503 4-甲氧基氯化三苯甲烷 4-Monomethoxytrityl chloride 97% 14470-28-1 5g,10g,25g,100g,250g
B104818  苯甲酸酐 Benzoic anhydride 98% 93-97-0 25g,100g,500g

脱保护剂

产品号 中文名称 英文名称 级别 Cas 规格
T103291 三氟乙酸(TFA) Trifluoroacetic acid AR,99.0% 76-05-1 100g,500g,2.5kg
H107501 六氟异丙醇 1,1,1,3,3,3-Hexafluoro-2-propanol 99.50% 920-66-1  25g,100g,250g,500g,2.5kg
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